mouse saa Search Results


mouse elisa kits  (Multi Sciences (Lianke) Biotech Co Ltd)
90
Multi Sciences (Lianke) Biotech Co Ltd mouse elisa kits
NCAM1 overexpression in microglia/macrophages confers protection against neuroinflammation and apoptotic cell death after ischemic stroke in mouse tMCAO model. A Quantification for IL-1β, <t>IL-6,</t> <t>TNF-α,</t> CXCL1, and CCL2 mRNA expression in the peri-infarct region of CX3CR1 Cre/ERT2 mice by qRT-PCR. n = 4. Results are represented as means ± SD. *** p < 0.001 vs. sham group and ## p < 0.01 and ### p < 0.001 vs. tMCAO plus Vector group. B Quantification for IL-1β, IL-6, TNF-α, CXCL1, and CCL2 protein expression in the peri-infarct region of CX3CR1 Cre/ERT2 mice by <t>ELISA.</t> n = 4. Results are represented as means ± SD. *** p < 0.001 vs. sham group and ### p < 0.001 vs. tMCAO plus Vector group. C and D Western blotting images and quantitative analysis for iNOS, CD32, and CD86 protein expression in the peri-infarct region of CX3CR1 Cre/ERT2 mice. n = 4. *** p < 0.001 vs. sham group and ### p < 0.001 vs. tMCAO plus Vector group. E and F Western blotting images and quantitative analysis for Bcl-xl, Bax, cleaved caspase-3, −9, and PARP protein expression in the peri-infarct region of CX3CR1 Cre/ERT2 mice. n = 4. *** p < 0.001 vs. sham group and ### p < 0.001 vs. tMCAO plus Vector group. G and H Representative immunofluorescence images and quantitative analysis for TUNEL-positive cells in the peri-infarct hippocampus and cortex of CX3CR1 Cre/ERT2 mice by TUNEL staining. n = 4. Scale bar: 50 μm. *** p < 0.001 vs. sham group and ### p < 0.001 vs. tMCAO plus Vector group
Mouse Elisa Kits, supplied by Multi Sciences (Lianke) Biotech Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
mouse elisa kits - by Bioz Stars, 2026-07
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saa3  (OriGene)
93
OriGene saa3
NF-κB activation in RAW cells overexpressing SAA proteins was assessed in response to stimulation with various immune ligands, or treatment with purified SAA proteins, compared to the response to LPS in control RAW cells. RAW-Blue™ cells (RAW 264.7) overexpressing SAA proteins were treated with a single dose of LPS, IL-6, TNFα, FSL-1, Pam3CSK4, FLAP2, R-848, Poly(I), ODN2395, C12-iE-DAP, MDP, Poly dT, LTA-SA, Zymosan, and Peptidoglycan overnight to activate NF-κB. Non-stimulated cells served as negative controls. (A) Heatmap showing NF-κB activity in RAW cells overexpressing SAA proteins (SAA1, SAA2, <t>SAA3,</t> SAA4) normalized against control RAW cells, with log2-transformed absorbance values (@600nm) displayed. (B) Representative bar graph of NF-κB activity in response to LPS stimulation in RAW cells overexpressing DDK-tagged SAA proteins (SAA1-DDK to SAA4-DDK) compared to unmodified RAW cells (n = 3). (C) NF-κB activity in RAW cells treated with purified SAA proteins (SAA1-100ng/ml, SAA2-10ng/ml, SAA3-10ng/ml, SAA4-100ng/ml) in the absence or presence of LPS (n = 3). Data represent mean ± SEM. Statistical significance is denoted as *(p < 0.05), **(p < 0.01), ***(p < 0.001), and ****(p < 0.0001). (ns = non-significant).
Saa3, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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saa3 - by Bioz Stars, 2026-07
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saa1 mouse elisa kit  (R&D Systems)
90
R&D Systems saa1 mouse elisa kit
NF-κB activation in RAW cells overexpressing SAA proteins was assessed in response to stimulation with various immune ligands, or treatment with purified SAA proteins, compared to the response to LPS in control RAW cells. RAW-Blue™ cells (RAW 264.7) overexpressing SAA proteins were treated with a single dose of LPS, IL-6, TNFα, FSL-1, Pam3CSK4, FLAP2, R-848, Poly(I), ODN2395, C12-iE-DAP, MDP, Poly dT, LTA-SA, Zymosan, and Peptidoglycan overnight to activate NF-κB. Non-stimulated cells served as negative controls. (A) Heatmap showing NF-κB activity in RAW cells overexpressing SAA proteins (SAA1, SAA2, <t>SAA3,</t> SAA4) normalized against control RAW cells, with log2-transformed absorbance values (@600nm) displayed. (B) Representative bar graph of NF-κB activity in response to LPS stimulation in RAW cells overexpressing DDK-tagged SAA proteins (SAA1-DDK to SAA4-DDK) compared to unmodified RAW cells (n = 3). (C) NF-κB activity in RAW cells treated with purified SAA proteins (SAA1-100ng/ml, SAA2-10ng/ml, SAA3-10ng/ml, SAA4-100ng/ml) in the absence or presence of LPS (n = 3). Data represent mean ± SEM. Statistical significance is denoted as *(p < 0.05), **(p < 0.01), ***(p < 0.001), and ****(p < 0.0001). (ns = non-significant).
Saa1 Mouse Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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saa4  (OriGene)
93
OriGene saa4
NF-κB activation in RAW cells overexpressing SAA proteins was assessed in response to stimulation with various immune ligands, or treatment with purified SAA proteins, compared to the response to LPS in control RAW cells. RAW-Blue™ cells (RAW 264.7) overexpressing SAA proteins were treated with a single dose of LPS, IL-6, TNFα, FSL-1, Pam3CSK4, FLAP2, R-848, Poly(I), ODN2395, C12-iE-DAP, MDP, Poly dT, LTA-SA, Zymosan, and Peptidoglycan overnight to activate NF-κB. Non-stimulated cells served as negative controls. (A) Heatmap showing NF-κB activity in RAW cells overexpressing SAA proteins (SAA1, SAA2, SAA3, <t>SAA4)</t> normalized against control RAW cells, with log2-transformed absorbance values (@600nm) displayed. (B) Representative bar graph of NF-κB activity in response to LPS stimulation in RAW cells overexpressing DDK-tagged SAA proteins (SAA1-DDK to <t>SAA4-DDK)</t> compared to unmodified RAW cells (n = 3). (C) NF-κB activity in RAW cells treated with purified SAA proteins (SAA1-100ng/ml, SAA2-10ng/ml, SAA3-10ng/ml, SAA4-100ng/ml) in the absence or presence of LPS (n = 3). Data represent mean ± SEM. Statistical significance is denoted as *(p < 0.05), **(p < 0.01), ***(p < 0.001), and ****(p < 0.0001). (ns = non-significant).
Saa4, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+saa/pmc11885894-57-32-43?v=OriGene
Average 93 stars, based on 1 article reviews
saa4 - by Bioz Stars, 2026-07
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saa2  (OriGene)
93
OriGene saa2
NF-κB activation in RAW cells overexpressing SAA proteins was assessed in response to stimulation with various immune ligands, or treatment with purified SAA proteins, compared to the response to LPS in control RAW cells. RAW-Blue™ cells (RAW 264.7) overexpressing SAA proteins were treated with a single dose of LPS, IL-6, TNFα, FSL-1, Pam3CSK4, FLAP2, R-848, Poly(I), ODN2395, C12-iE-DAP, MDP, Poly dT, LTA-SA, Zymosan, and Peptidoglycan overnight to activate NF-κB. Non-stimulated cells served as negative controls. (A) Heatmap showing NF-κB activity in RAW cells overexpressing SAA proteins (SAA1, <t>SAA2,</t> SAA3, SAA4) normalized against control RAW cells, with log2-transformed absorbance values (@600nm) displayed. (B) Representative bar graph of NF-κB activity in response to LPS stimulation in RAW cells overexpressing DDK-tagged SAA proteins (SAA1-DDK to SAA4-DDK) compared to unmodified RAW cells (n = 3). (C) NF-κB activity in RAW cells treated with purified SAA proteins (SAA1-100ng/ml, SAA2-10ng/ml, SAA3-10ng/ml, SAA4-100ng/ml) in the absence or presence of LPS (n = 3). Data represent mean ± SEM. Statistical significance is denoted as *(p < 0.05), **(p < 0.01), ***(p < 0.001), and ****(p < 0.0001). (ns = non-significant).
Saa2, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+saa/pmc11885894-57-15-43?v=OriGene
Average 93 stars, based on 1 article reviews
saa2 - by Bioz Stars, 2026-07
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elisa kits  (Aviva Systems)
93
Aviva Systems elisa kits
NF-κB activation in RAW cells overexpressing SAA proteins was assessed in response to stimulation with various immune ligands, or treatment with purified SAA proteins, compared to the response to LPS in control RAW cells. RAW-Blue™ cells (RAW 264.7) overexpressing SAA proteins were treated with a single dose of LPS, IL-6, TNFα, FSL-1, Pam3CSK4, FLAP2, R-848, Poly(I), ODN2395, C12-iE-DAP, MDP, Poly dT, LTA-SA, Zymosan, and Peptidoglycan overnight to activate NF-κB. Non-stimulated cells served as negative controls. (A) Heatmap showing NF-κB activity in RAW cells overexpressing SAA proteins (SAA1, <t>SAA2,</t> SAA3, SAA4) normalized against control RAW cells, with log2-transformed absorbance values (@600nm) displayed. (B) Representative bar graph of NF-κB activity in response to LPS stimulation in RAW cells overexpressing DDK-tagged SAA proteins (SAA1-DDK to SAA4-DDK) compared to unmodified RAW cells (n = 3). (C) NF-κB activity in RAW cells treated with purified SAA proteins (SAA1-100ng/ml, SAA2-10ng/ml, SAA3-10ng/ml, SAA4-100ng/ml) in the absence or presence of LPS (n = 3). Data represent mean ± SEM. Statistical significance is denoted as *(p < 0.05), **(p < 0.01), ***(p < 0.001), and ****(p < 0.0001). (ns = non-significant).
Elisa Kits, supplied by Aviva Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+saa/pm39947611-71-14-18?v=Aviva+Systems
Average 93 stars, based on 1 article reviews
elisa kits - by Bioz Stars, 2026-07
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myc ddk tags for saa1  (OriGene)
93
OriGene myc ddk tags for saa1
NF-κB activation in RAW cells overexpressing SAA proteins was assessed in response to stimulation with various immune ligands, or treatment with purified SAA proteins, compared to the response to LPS in control RAW cells. RAW-Blue™ cells (RAW 264.7) overexpressing SAA proteins were treated with a single dose of LPS, IL-6, TNFα, FSL-1, Pam3CSK4, FLAP2, R-848, Poly(I), ODN2395, C12-iE-DAP, MDP, Poly dT, LTA-SA, Zymosan, and Peptidoglycan overnight to activate NF-κB. Non-stimulated cells served as negative controls. (A) Heatmap showing NF-κB activity in RAW cells overexpressing SAA proteins <t>(SAA1,</t> SAA2, SAA3, SAA4) normalized against control RAW cells, with log2-transformed absorbance values (@600nm) displayed. (B) Representative bar graph of NF-κB activity in response to LPS stimulation in RAW cells overexpressing DDK-tagged SAA proteins (SAA1-DDK to SAA4-DDK) compared to unmodified RAW cells (n = 3). (C) NF-κB activity in RAW cells treated with purified SAA proteins (SAA1-100ng/ml, SAA2-10ng/ml, SAA3-10ng/ml, SAA4-100ng/ml) in the absence or presence of LPS (n = 3). Data represent mean ± SEM. Statistical significance is denoted as *(p < 0.05), **(p < 0.01), ***(p < 0.001), and ****(p < 0.0001). (ns = non-significant).
Myc Ddk Tags For Saa1, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+saa/pmc11885894-57-4-43?v=OriGene
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mouse serum elisa quantitation kit  (Elabscience Biotechnology)
93
Elabscience Biotechnology mouse serum elisa quantitation kit
Overexpression of CHRFAM7A reduces unilateral ureteral obstruction (UUO)‐induced inflammatory cytokines and grow factors. (A, B) IL‐6 levels in serum and kidney were measured by <t>ELISA</t> ( n = 5). All values are expressed as mean ± SD. (C–H) The expression of inflammatory factors IL‐1β, IL‐6, TNF‐α, CCL2, CD206 and FIZZ1in mouse kidney was detected by q‐PCR. Up‐regulation of CHRFAM7A reduced mRNA expression of IL‐1β, IL‐6, TNF‐α and CCL2 in response to UUO mice but increased the expression of CD206 and FIZZ1 compared with WT‐UUO mice ( n = 5). ** p < 0.01 vs. WT group; # p < 0.05, ## p < 0.01 vs. WT‐UUO group.
Mouse Serum Elisa Quantitation Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+saa/pmc09806291-49-7-17?v=Elabscience+Biotechnology
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mouse serum elisa quantitation kit - by Bioz Stars, 2026-07
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mouse saa elisa kit  (Boster Bio)
92
Boster Bio mouse saa elisa kit
Overexpression of CHRFAM7A reduces unilateral ureteral obstruction (UUO)‐induced inflammatory cytokines and grow factors. (A, B) IL‐6 levels in serum and kidney were measured by <t>ELISA</t> ( n = 5). All values are expressed as mean ± SD. (C–H) The expression of inflammatory factors IL‐1β, IL‐6, TNF‐α, CCL2, CD206 and FIZZ1in mouse kidney was detected by q‐PCR. Up‐regulation of CHRFAM7A reduced mRNA expression of IL‐1β, IL‐6, TNF‐α and CCL2 in response to UUO mice but increased the expression of CD206 and FIZZ1 compared with WT‐UUO mice ( n = 5). ** p < 0.01 vs. WT group; # p < 0.05, ## p < 0.01 vs. WT‐UUO group.
Mouse Saa Elisa Kit, supplied by Boster Bio, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse serum inclusion elisa kit  (Cusabio)
90
Cusabio mouse serum inclusion elisa kit
Overexpression of CHRFAM7A reduces unilateral ureteral obstruction (UUO)‐induced inflammatory cytokines and grow factors. (A, B) IL‐6 levels in serum and kidney were measured by <t>ELISA</t> ( n = 5). All values are expressed as mean ± SD. (C–H) The expression of inflammatory factors IL‐1β, IL‐6, TNF‐α, CCL2, CD206 and FIZZ1in mouse kidney was detected by q‐PCR. Up‐regulation of CHRFAM7A reduced mRNA expression of IL‐1β, IL‐6, TNF‐α and CCL2 in response to UUO mice but increased the expression of CD206 and FIZZ1 compared with WT‐UUO mice ( n = 5). ** p < 0.01 vs. WT group; # p < 0.05, ## p < 0.01 vs. WT‐UUO group.
Mouse Serum Inclusion Elisa Kit, supplied by Cusabio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse serum  (Eagle Biosciences)
85
Eagle Biosciences mouse serum
Overexpression of CHRFAM7A reduces unilateral ureteral obstruction (UUO)‐induced inflammatory cytokines and grow factors. (A, B) IL‐6 levels in serum and kidney were measured by <t>ELISA</t> ( n = 5). All values are expressed as mean ± SD. (C–H) The expression of inflammatory factors IL‐1β, IL‐6, TNF‐α, CCL2, CD206 and FIZZ1in mouse kidney was detected by q‐PCR. Up‐regulation of CHRFAM7A reduced mRNA expression of IL‐1β, IL‐6, TNF‐α and CCL2 in response to UUO mice but increased the expression of CD206 and FIZZ1 compared with WT‐UUO mice ( n = 5). ** p < 0.01 vs. WT group; # p < 0.05, ## p < 0.01 vs. WT‐UUO group.
Mouse Serum, supplied by Eagle Biosciences, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse saa kit  (Abnova)
90
Abnova mouse saa kit
Overexpression of CHRFAM7A reduces unilateral ureteral obstruction (UUO)‐induced inflammatory cytokines and grow factors. (A, B) IL‐6 levels in serum and kidney were measured by <t>ELISA</t> ( n = 5). All values are expressed as mean ± SD. (C–H) The expression of inflammatory factors IL‐1β, IL‐6, TNF‐α, CCL2, CD206 and FIZZ1in mouse kidney was detected by q‐PCR. Up‐regulation of CHRFAM7A reduced mRNA expression of IL‐1β, IL‐6, TNF‐α and CCL2 in response to UUO mice but increased the expression of CD206 and FIZZ1 compared with WT‐UUO mice ( n = 5). ** p < 0.01 vs. WT group; # p < 0.05, ## p < 0.01 vs. WT‐UUO group.
Mouse Saa Kit, supplied by Abnova, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


NCAM1 overexpression in microglia/macrophages confers protection against neuroinflammation and apoptotic cell death after ischemic stroke in mouse tMCAO model. A Quantification for IL-1β, IL-6, TNF-α, CXCL1, and CCL2 mRNA expression in the peri-infarct region of CX3CR1 Cre/ERT2 mice by qRT-PCR. n = 4. Results are represented as means ± SD. *** p < 0.001 vs. sham group and ## p < 0.01 and ### p < 0.001 vs. tMCAO plus Vector group. B Quantification for IL-1β, IL-6, TNF-α, CXCL1, and CCL2 protein expression in the peri-infarct region of CX3CR1 Cre/ERT2 mice by ELISA. n = 4. Results are represented as means ± SD. *** p < 0.001 vs. sham group and ### p < 0.001 vs. tMCAO plus Vector group. C and D Western blotting images and quantitative analysis for iNOS, CD32, and CD86 protein expression in the peri-infarct region of CX3CR1 Cre/ERT2 mice. n = 4. *** p < 0.001 vs. sham group and ### p < 0.001 vs. tMCAO plus Vector group. E and F Western blotting images and quantitative analysis for Bcl-xl, Bax, cleaved caspase-3, −9, and PARP protein expression in the peri-infarct region of CX3CR1 Cre/ERT2 mice. n = 4. *** p < 0.001 vs. sham group and ### p < 0.001 vs. tMCAO plus Vector group. G and H Representative immunofluorescence images and quantitative analysis for TUNEL-positive cells in the peri-infarct hippocampus and cortex of CX3CR1 Cre/ERT2 mice by TUNEL staining. n = 4. Scale bar: 50 μm. *** p < 0.001 vs. sham group and ### p < 0.001 vs. tMCAO plus Vector group

Journal: Journal of Neuroinflammation

Article Title: Microglial NCAM1 attenuates ischemic brain injury by inhibiting NF-κB-driven neuroinflammation through IκBα stabilization

doi: 10.1186/s12974-026-03766-7

Figure Lengend Snippet: NCAM1 overexpression in microglia/macrophages confers protection against neuroinflammation and apoptotic cell death after ischemic stroke in mouse tMCAO model. A Quantification for IL-1β, IL-6, TNF-α, CXCL1, and CCL2 mRNA expression in the peri-infarct region of CX3CR1 Cre/ERT2 mice by qRT-PCR. n = 4. Results are represented as means ± SD. *** p < 0.001 vs. sham group and ## p < 0.01 and ### p < 0.001 vs. tMCAO plus Vector group. B Quantification for IL-1β, IL-6, TNF-α, CXCL1, and CCL2 protein expression in the peri-infarct region of CX3CR1 Cre/ERT2 mice by ELISA. n = 4. Results are represented as means ± SD. *** p < 0.001 vs. sham group and ### p < 0.001 vs. tMCAO plus Vector group. C and D Western blotting images and quantitative analysis for iNOS, CD32, and CD86 protein expression in the peri-infarct region of CX3CR1 Cre/ERT2 mice. n = 4. *** p < 0.001 vs. sham group and ### p < 0.001 vs. tMCAO plus Vector group. E and F Western blotting images and quantitative analysis for Bcl-xl, Bax, cleaved caspase-3, −9, and PARP protein expression in the peri-infarct region of CX3CR1 Cre/ERT2 mice. n = 4. *** p < 0.001 vs. sham group and ### p < 0.001 vs. tMCAO plus Vector group. G and H Representative immunofluorescence images and quantitative analysis for TUNEL-positive cells in the peri-infarct hippocampus and cortex of CX3CR1 Cre/ERT2 mice by TUNEL staining. n = 4. Scale bar: 50 μm. *** p < 0.001 vs. sham group and ### p < 0.001 vs. tMCAO plus Vector group

Article Snippet: The levels of IL-1β, IL-6, TNF-α, CXCL1, and CCL2 in tissue homogenates were quantified using commercial mouse ELISA kits (Multi Sciences, Hangzhou, China), and the results were expressed as picograms per milliliter (pg/mL).

Techniques: Over Expression, Expressing, Quantitative RT-PCR, Plasmid Preparation, Enzyme-linked Immunosorbent Assay, Western Blot, Immunofluorescence, TUNEL Assay, Staining

NF-κB activation in RAW cells overexpressing SAA proteins was assessed in response to stimulation with various immune ligands, or treatment with purified SAA proteins, compared to the response to LPS in control RAW cells. RAW-Blue™ cells (RAW 264.7) overexpressing SAA proteins were treated with a single dose of LPS, IL-6, TNFα, FSL-1, Pam3CSK4, FLAP2, R-848, Poly(I), ODN2395, C12-iE-DAP, MDP, Poly dT, LTA-SA, Zymosan, and Peptidoglycan overnight to activate NF-κB. Non-stimulated cells served as negative controls. (A) Heatmap showing NF-κB activity in RAW cells overexpressing SAA proteins (SAA1, SAA2, SAA3, SAA4) normalized against control RAW cells, with log2-transformed absorbance values (@600nm) displayed. (B) Representative bar graph of NF-κB activity in response to LPS stimulation in RAW cells overexpressing DDK-tagged SAA proteins (SAA1-DDK to SAA4-DDK) compared to unmodified RAW cells (n = 3). (C) NF-κB activity in RAW cells treated with purified SAA proteins (SAA1-100ng/ml, SAA2-10ng/ml, SAA3-10ng/ml, SAA4-100ng/ml) in the absence or presence of LPS (n = 3). Data represent mean ± SEM. Statistical significance is denoted as *(p < 0.05), **(p < 0.01), ***(p < 0.001), and ****(p < 0.0001). (ns = non-significant).

Journal: Frontiers in Immunology

Article Title: Balancing inflammation: the specific roles of serum amyloid A proteins in sterile and infectious diseases

doi: 10.3389/fimmu.2025.1544085

Figure Lengend Snippet: NF-κB activation in RAW cells overexpressing SAA proteins was assessed in response to stimulation with various immune ligands, or treatment with purified SAA proteins, compared to the response to LPS in control RAW cells. RAW-Blue™ cells (RAW 264.7) overexpressing SAA proteins were treated with a single dose of LPS, IL-6, TNFα, FSL-1, Pam3CSK4, FLAP2, R-848, Poly(I), ODN2395, C12-iE-DAP, MDP, Poly dT, LTA-SA, Zymosan, and Peptidoglycan overnight to activate NF-κB. Non-stimulated cells served as negative controls. (A) Heatmap showing NF-κB activity in RAW cells overexpressing SAA proteins (SAA1, SAA2, SAA3, SAA4) normalized against control RAW cells, with log2-transformed absorbance values (@600nm) displayed. (B) Representative bar graph of NF-κB activity in response to LPS stimulation in RAW cells overexpressing DDK-tagged SAA proteins (SAA1-DDK to SAA4-DDK) compared to unmodified RAW cells (n = 3). (C) NF-κB activity in RAW cells treated with purified SAA proteins (SAA1-100ng/ml, SAA2-10ng/ml, SAA3-10ng/ml, SAA4-100ng/ml) in the absence or presence of LPS (n = 3). Data represent mean ± SEM. Statistical significance is denoted as *(p < 0.05), **(p < 0.01), ***(p < 0.001), and ****(p < 0.0001). (ns = non-significant).

Article Snippet: The expression plasmids with Myc-DDK tags for SAA1 (MR200611, Saa1 (NM_009117) Mouse Tagged ORF Clone), SAA2 (MR219150, Saa2 (NM_011314) Mouse Tagged ORF Clone), SAA3 (MR200605, Saa3 (NM_011315) Mouse Tagged ORF Clone), and SAA4 (MR200725, Saa4 (NM_011316) Mouse Tagged ORF Clone) were purchased from Origene Technologies.

Techniques: Activation Assay, Purification, Control, Activity Assay, Transformation Assay

Concentrations of SAA proteins in response to LPS and P. aeruginosa infection. Concentrations of SAA1, SAA2, SAA3, and SAA4 were measured in samples from Healthy, LPS-treated, and P. aeruginosa -infected conditions (Healthy n = 6; LPS n=8; and P. aeruginosa n=10). Data represent mean ± SEM. Statistical significance is indicated by * (p < 0.05), ** (p < 0.01), *** (p < 0.001), and **** (p < 0.0001). (ns = non-significant).

Journal: Frontiers in Immunology

Article Title: Balancing inflammation: the specific roles of serum amyloid A proteins in sterile and infectious diseases

doi: 10.3389/fimmu.2025.1544085

Figure Lengend Snippet: Concentrations of SAA proteins in response to LPS and P. aeruginosa infection. Concentrations of SAA1, SAA2, SAA3, and SAA4 were measured in samples from Healthy, LPS-treated, and P. aeruginosa -infected conditions (Healthy n = 6; LPS n=8; and P. aeruginosa n=10). Data represent mean ± SEM. Statistical significance is indicated by * (p < 0.05), ** (p < 0.01), *** (p < 0.001), and **** (p < 0.0001). (ns = non-significant).

Article Snippet: The expression plasmids with Myc-DDK tags for SAA1 (MR200611, Saa1 (NM_009117) Mouse Tagged ORF Clone), SAA2 (MR219150, Saa2 (NM_011314) Mouse Tagged ORF Clone), SAA3 (MR200605, Saa3 (NM_011315) Mouse Tagged ORF Clone), and SAA4 (MR200725, Saa4 (NM_011316) Mouse Tagged ORF Clone) were purchased from Origene Technologies.

Techniques: Infection

NF-κB activation in RAW cells overexpressing SAA proteins was assessed in response to stimulation with various immune ligands, or treatment with purified SAA proteins, compared to the response to LPS in control RAW cells. RAW-Blue™ cells (RAW 264.7) overexpressing SAA proteins were treated with a single dose of LPS, IL-6, TNFα, FSL-1, Pam3CSK4, FLAP2, R-848, Poly(I), ODN2395, C12-iE-DAP, MDP, Poly dT, LTA-SA, Zymosan, and Peptidoglycan overnight to activate NF-κB. Non-stimulated cells served as negative controls. (A) Heatmap showing NF-κB activity in RAW cells overexpressing SAA proteins (SAA1, SAA2, SAA3, SAA4) normalized against control RAW cells, with log2-transformed absorbance values (@600nm) displayed. (B) Representative bar graph of NF-κB activity in response to LPS stimulation in RAW cells overexpressing DDK-tagged SAA proteins (SAA1-DDK to SAA4-DDK) compared to unmodified RAW cells (n = 3). (C) NF-κB activity in RAW cells treated with purified SAA proteins (SAA1-100ng/ml, SAA2-10ng/ml, SAA3-10ng/ml, SAA4-100ng/ml) in the absence or presence of LPS (n = 3). Data represent mean ± SEM. Statistical significance is denoted as *(p < 0.05), **(p < 0.01), ***(p < 0.001), and ****(p < 0.0001). (ns = non-significant).

Journal: Frontiers in Immunology

Article Title: Balancing inflammation: the specific roles of serum amyloid A proteins in sterile and infectious diseases

doi: 10.3389/fimmu.2025.1544085

Figure Lengend Snippet: NF-κB activation in RAW cells overexpressing SAA proteins was assessed in response to stimulation with various immune ligands, or treatment with purified SAA proteins, compared to the response to LPS in control RAW cells. RAW-Blue™ cells (RAW 264.7) overexpressing SAA proteins were treated with a single dose of LPS, IL-6, TNFα, FSL-1, Pam3CSK4, FLAP2, R-848, Poly(I), ODN2395, C12-iE-DAP, MDP, Poly dT, LTA-SA, Zymosan, and Peptidoglycan overnight to activate NF-κB. Non-stimulated cells served as negative controls. (A) Heatmap showing NF-κB activity in RAW cells overexpressing SAA proteins (SAA1, SAA2, SAA3, SAA4) normalized against control RAW cells, with log2-transformed absorbance values (@600nm) displayed. (B) Representative bar graph of NF-κB activity in response to LPS stimulation in RAW cells overexpressing DDK-tagged SAA proteins (SAA1-DDK to SAA4-DDK) compared to unmodified RAW cells (n = 3). (C) NF-κB activity in RAW cells treated with purified SAA proteins (SAA1-100ng/ml, SAA2-10ng/ml, SAA3-10ng/ml, SAA4-100ng/ml) in the absence or presence of LPS (n = 3). Data represent mean ± SEM. Statistical significance is denoted as *(p < 0.05), **(p < 0.01), ***(p < 0.001), and ****(p < 0.0001). (ns = non-significant).

Article Snippet: The expression plasmids with Myc-DDK tags for SAA1 (MR200611, Saa1 (NM_009117) Mouse Tagged ORF Clone), SAA2 (MR219150, Saa2 (NM_011314) Mouse Tagged ORF Clone), SAA3 (MR200605, Saa3 (NM_011315) Mouse Tagged ORF Clone), and SAA4 (MR200725, Saa4 (NM_011316) Mouse Tagged ORF Clone) were purchased from Origene Technologies.

Techniques: Activation Assay, Purification, Control, Activity Assay, Transformation Assay

Concentrations of SAA proteins in response to LPS and P. aeruginosa infection. Concentrations of SAA1, SAA2, SAA3, and SAA4 were measured in samples from Healthy, LPS-treated, and P. aeruginosa -infected conditions (Healthy n = 6; LPS n=8; and P. aeruginosa n=10). Data represent mean ± SEM. Statistical significance is indicated by * (p < 0.05), ** (p < 0.01), *** (p < 0.001), and **** (p < 0.0001). (ns = non-significant).

Journal: Frontiers in Immunology

Article Title: Balancing inflammation: the specific roles of serum amyloid A proteins in sterile and infectious diseases

doi: 10.3389/fimmu.2025.1544085

Figure Lengend Snippet: Concentrations of SAA proteins in response to LPS and P. aeruginosa infection. Concentrations of SAA1, SAA2, SAA3, and SAA4 were measured in samples from Healthy, LPS-treated, and P. aeruginosa -infected conditions (Healthy n = 6; LPS n=8; and P. aeruginosa n=10). Data represent mean ± SEM. Statistical significance is indicated by * (p < 0.05), ** (p < 0.01), *** (p < 0.001), and **** (p < 0.0001). (ns = non-significant).

Article Snippet: The expression plasmids with Myc-DDK tags for SAA1 (MR200611, Saa1 (NM_009117) Mouse Tagged ORF Clone), SAA2 (MR219150, Saa2 (NM_011314) Mouse Tagged ORF Clone), SAA3 (MR200605, Saa3 (NM_011315) Mouse Tagged ORF Clone), and SAA4 (MR200725, Saa4 (NM_011316) Mouse Tagged ORF Clone) were purchased from Origene Technologies.

Techniques: Infection

NF-κB activation in RAW cells overexpressing SAA proteins was assessed in response to stimulation with various immune ligands, or treatment with purified SAA proteins, compared to the response to LPS in control RAW cells. RAW-Blue™ cells (RAW 264.7) overexpressing SAA proteins were treated with a single dose of LPS, IL-6, TNFα, FSL-1, Pam3CSK4, FLAP2, R-848, Poly(I), ODN2395, C12-iE-DAP, MDP, Poly dT, LTA-SA, Zymosan, and Peptidoglycan overnight to activate NF-κB. Non-stimulated cells served as negative controls. (A) Heatmap showing NF-κB activity in RAW cells overexpressing SAA proteins (SAA1, SAA2, SAA3, SAA4) normalized against control RAW cells, with log2-transformed absorbance values (@600nm) displayed. (B) Representative bar graph of NF-κB activity in response to LPS stimulation in RAW cells overexpressing DDK-tagged SAA proteins (SAA1-DDK to SAA4-DDK) compared to unmodified RAW cells (n = 3). (C) NF-κB activity in RAW cells treated with purified SAA proteins (SAA1-100ng/ml, SAA2-10ng/ml, SAA3-10ng/ml, SAA4-100ng/ml) in the absence or presence of LPS (n = 3). Data represent mean ± SEM. Statistical significance is denoted as *(p < 0.05), **(p < 0.01), ***(p < 0.001), and ****(p < 0.0001). (ns = non-significant).

Journal: Frontiers in Immunology

Article Title: Balancing inflammation: the specific roles of serum amyloid A proteins in sterile and infectious diseases

doi: 10.3389/fimmu.2025.1544085

Figure Lengend Snippet: NF-κB activation in RAW cells overexpressing SAA proteins was assessed in response to stimulation with various immune ligands, or treatment with purified SAA proteins, compared to the response to LPS in control RAW cells. RAW-Blue™ cells (RAW 264.7) overexpressing SAA proteins were treated with a single dose of LPS, IL-6, TNFα, FSL-1, Pam3CSK4, FLAP2, R-848, Poly(I), ODN2395, C12-iE-DAP, MDP, Poly dT, LTA-SA, Zymosan, and Peptidoglycan overnight to activate NF-κB. Non-stimulated cells served as negative controls. (A) Heatmap showing NF-κB activity in RAW cells overexpressing SAA proteins (SAA1, SAA2, SAA3, SAA4) normalized against control RAW cells, with log2-transformed absorbance values (@600nm) displayed. (B) Representative bar graph of NF-κB activity in response to LPS stimulation in RAW cells overexpressing DDK-tagged SAA proteins (SAA1-DDK to SAA4-DDK) compared to unmodified RAW cells (n = 3). (C) NF-κB activity in RAW cells treated with purified SAA proteins (SAA1-100ng/ml, SAA2-10ng/ml, SAA3-10ng/ml, SAA4-100ng/ml) in the absence or presence of LPS (n = 3). Data represent mean ± SEM. Statistical significance is denoted as *(p < 0.05), **(p < 0.01), ***(p < 0.001), and ****(p < 0.0001). (ns = non-significant).

Article Snippet: The expression plasmids with Myc-DDK tags for SAA1 (MR200611, Saa1 (NM_009117) Mouse Tagged ORF Clone), SAA2 (MR219150, Saa2 (NM_011314) Mouse Tagged ORF Clone), SAA3 (MR200605, Saa3 (NM_011315) Mouse Tagged ORF Clone), and SAA4 (MR200725, Saa4 (NM_011316) Mouse Tagged ORF Clone) were purchased from Origene Technologies.

Techniques: Activation Assay, Purification, Control, Activity Assay, Transformation Assay

Concentrations of SAA proteins in response to LPS and P. aeruginosa infection. Concentrations of SAA1, SAA2, SAA3, and SAA4 were measured in samples from Healthy, LPS-treated, and P. aeruginosa -infected conditions (Healthy n = 6; LPS n=8; and P. aeruginosa n=10). Data represent mean ± SEM. Statistical significance is indicated by * (p < 0.05), ** (p < 0.01), *** (p < 0.001), and **** (p < 0.0001). (ns = non-significant).

Journal: Frontiers in Immunology

Article Title: Balancing inflammation: the specific roles of serum amyloid A proteins in sterile and infectious diseases

doi: 10.3389/fimmu.2025.1544085

Figure Lengend Snippet: Concentrations of SAA proteins in response to LPS and P. aeruginosa infection. Concentrations of SAA1, SAA2, SAA3, and SAA4 were measured in samples from Healthy, LPS-treated, and P. aeruginosa -infected conditions (Healthy n = 6; LPS n=8; and P. aeruginosa n=10). Data represent mean ± SEM. Statistical significance is indicated by * (p < 0.05), ** (p < 0.01), *** (p < 0.001), and **** (p < 0.0001). (ns = non-significant).

Article Snippet: The expression plasmids with Myc-DDK tags for SAA1 (MR200611, Saa1 (NM_009117) Mouse Tagged ORF Clone), SAA2 (MR219150, Saa2 (NM_011314) Mouse Tagged ORF Clone), SAA3 (MR200605, Saa3 (NM_011315) Mouse Tagged ORF Clone), and SAA4 (MR200725, Saa4 (NM_011316) Mouse Tagged ORF Clone) were purchased from Origene Technologies.

Techniques: Infection

NF-κB activation in RAW cells overexpressing SAA proteins was assessed in response to stimulation with various immune ligands, or treatment with purified SAA proteins, compared to the response to LPS in control RAW cells. RAW-Blue™ cells (RAW 264.7) overexpressing SAA proteins were treated with a single dose of LPS, IL-6, TNFα, FSL-1, Pam3CSK4, FLAP2, R-848, Poly(I), ODN2395, C12-iE-DAP, MDP, Poly dT, LTA-SA, Zymosan, and Peptidoglycan overnight to activate NF-κB. Non-stimulated cells served as negative controls. (A) Heatmap showing NF-κB activity in RAW cells overexpressing SAA proteins (SAA1, SAA2, SAA3, SAA4) normalized against control RAW cells, with log2-transformed absorbance values (@600nm) displayed. (B) Representative bar graph of NF-κB activity in response to LPS stimulation in RAW cells overexpressing DDK-tagged SAA proteins (SAA1-DDK to SAA4-DDK) compared to unmodified RAW cells (n = 3). (C) NF-κB activity in RAW cells treated with purified SAA proteins (SAA1-100ng/ml, SAA2-10ng/ml, SAA3-10ng/ml, SAA4-100ng/ml) in the absence or presence of LPS (n = 3). Data represent mean ± SEM. Statistical significance is denoted as *(p < 0.05), **(p < 0.01), ***(p < 0.001), and ****(p < 0.0001). (ns = non-significant).

Journal: Frontiers in Immunology

Article Title: Balancing inflammation: the specific roles of serum amyloid A proteins in sterile and infectious diseases

doi: 10.3389/fimmu.2025.1544085

Figure Lengend Snippet: NF-κB activation in RAW cells overexpressing SAA proteins was assessed in response to stimulation with various immune ligands, or treatment with purified SAA proteins, compared to the response to LPS in control RAW cells. RAW-Blue™ cells (RAW 264.7) overexpressing SAA proteins were treated with a single dose of LPS, IL-6, TNFα, FSL-1, Pam3CSK4, FLAP2, R-848, Poly(I), ODN2395, C12-iE-DAP, MDP, Poly dT, LTA-SA, Zymosan, and Peptidoglycan overnight to activate NF-κB. Non-stimulated cells served as negative controls. (A) Heatmap showing NF-κB activity in RAW cells overexpressing SAA proteins (SAA1, SAA2, SAA3, SAA4) normalized against control RAW cells, with log2-transformed absorbance values (@600nm) displayed. (B) Representative bar graph of NF-κB activity in response to LPS stimulation in RAW cells overexpressing DDK-tagged SAA proteins (SAA1-DDK to SAA4-DDK) compared to unmodified RAW cells (n = 3). (C) NF-κB activity in RAW cells treated with purified SAA proteins (SAA1-100ng/ml, SAA2-10ng/ml, SAA3-10ng/ml, SAA4-100ng/ml) in the absence or presence of LPS (n = 3). Data represent mean ± SEM. Statistical significance is denoted as *(p < 0.05), **(p < 0.01), ***(p < 0.001), and ****(p < 0.0001). (ns = non-significant).

Article Snippet: The expression plasmids with Myc-DDK tags for SAA1 (MR200611, Saa1 (NM_009117) Mouse Tagged ORF Clone), SAA2 (MR219150, Saa2 (NM_011314) Mouse Tagged ORF Clone), SAA3 (MR200605, Saa3 (NM_011315) Mouse Tagged ORF Clone), and SAA4 (MR200725, Saa4 (NM_011316) Mouse Tagged ORF Clone) were purchased from Origene Technologies.

Techniques: Activation Assay, Purification, Control, Activity Assay, Transformation Assay

Concentrations of SAA proteins in response to LPS and P. aeruginosa infection. Concentrations of SAA1, SAA2, SAA3, and SAA4 were measured in samples from Healthy, LPS-treated, and P. aeruginosa -infected conditions (Healthy n = 6; LPS n=8; and P. aeruginosa n=10). Data represent mean ± SEM. Statistical significance is indicated by * (p < 0.05), ** (p < 0.01), *** (p < 0.001), and **** (p < 0.0001). (ns = non-significant).

Journal: Frontiers in Immunology

Article Title: Balancing inflammation: the specific roles of serum amyloid A proteins in sterile and infectious diseases

doi: 10.3389/fimmu.2025.1544085

Figure Lengend Snippet: Concentrations of SAA proteins in response to LPS and P. aeruginosa infection. Concentrations of SAA1, SAA2, SAA3, and SAA4 were measured in samples from Healthy, LPS-treated, and P. aeruginosa -infected conditions (Healthy n = 6; LPS n=8; and P. aeruginosa n=10). Data represent mean ± SEM. Statistical significance is indicated by * (p < 0.05), ** (p < 0.01), *** (p < 0.001), and **** (p < 0.0001). (ns = non-significant).

Article Snippet: The expression plasmids with Myc-DDK tags for SAA1 (MR200611, Saa1 (NM_009117) Mouse Tagged ORF Clone), SAA2 (MR219150, Saa2 (NM_011314) Mouse Tagged ORF Clone), SAA3 (MR200605, Saa3 (NM_011315) Mouse Tagged ORF Clone), and SAA4 (MR200725, Saa4 (NM_011316) Mouse Tagged ORF Clone) were purchased from Origene Technologies.

Techniques: Infection

Overexpression of CHRFAM7A reduces unilateral ureteral obstruction (UUO)‐induced inflammatory cytokines and grow factors. (A, B) IL‐6 levels in serum and kidney were measured by ELISA ( n = 5). All values are expressed as mean ± SD. (C–H) The expression of inflammatory factors IL‐1β, IL‐6, TNF‐α, CCL2, CD206 and FIZZ1in mouse kidney was detected by q‐PCR. Up‐regulation of CHRFAM7A reduced mRNA expression of IL‐1β, IL‐6, TNF‐α and CCL2 in response to UUO mice but increased the expression of CD206 and FIZZ1 compared with WT‐UUO mice ( n = 5). ** p < 0.01 vs. WT group; # p < 0.05, ## p < 0.01 vs. WT‐UUO group.

Journal: Journal of Cellular and Molecular Medicine

Article Title: Up‐regulation of the human‐specific CHRFAM7A gene protects against renal fibrosis in mice with obstructive nephropathy

doi: 10.1111/jcmm.17630

Figure Lengend Snippet: Overexpression of CHRFAM7A reduces unilateral ureteral obstruction (UUO)‐induced inflammatory cytokines and grow factors. (A, B) IL‐6 levels in serum and kidney were measured by ELISA ( n = 5). All values are expressed as mean ± SD. (C–H) The expression of inflammatory factors IL‐1β, IL‐6, TNF‐α, CCL2, CD206 and FIZZ1in mouse kidney was detected by q‐PCR. Up‐regulation of CHRFAM7A reduced mRNA expression of IL‐1β, IL‐6, TNF‐α and CCL2 in response to UUO mice but increased the expression of CD206 and FIZZ1 compared with WT‐UUO mice ( n = 5). ** p < 0.01 vs. WT group; # p < 0.05, ## p < 0.01 vs. WT‐UUO group.

Article Snippet: Interleukin 6 (IL‐6) was measured using a mouse serum ELISA Quantitation kit, according to the manufacturer's protocol (Elabscience MM‐0163M2).

Techniques: Over Expression, Enzyme-linked Immunosorbent Assay, Expressing